Question

You look at 80 black and tan sordoria asci under the microscope and count 45 non-recombinantasci. What is the distance, in map units of the color gene from the centromere? Round to the nearest whole number, OOO QUESTION 4 What are the steps in a single PCR (Polymerase Chain Reaction) cycle? 1. Elongation II. Annealing III. Amplification IV. Denaturation V. Extension LILY IV, INI, LII IV. II. V IV,

Answer 1

The distance in map units of the color gene from the centromere is 22. The steps in single PCR are IV, II , V,

Calculations for the distance in map units can be calculated as follows:

Total no. of asci = 80

Number of non-recombinant asci = 45

Number of recombinant asci = 35

Recombination frequency = (35/80) x 100 % = 43.75%

Distance in map units = Recombination Frequency / 2  

= 43.75/2 = 21.875  

= 22

Polymerase chain reaction shortened as PCR is a laboratory method for hastily producing (amplifying) tens of thousands and thousands to billions of copies of a particular phase of DNA, which could then be studied in more detail. The 3 sequential steps of PCR are

a. Denaturation

b. Annealing

c. Extension

There are no steps in PCR called elongation and amplification.

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