Question

When using PCR to study DNA, the base pairs split and the DNA cleaves into single strands. What stage of the process does this MOST accurately describe?
A. annealing
B. denaturization
C. extension
D. hybridization

Answer 1

The stage of PCR being represented by the the splitting of base pairs and the cleaving of DNA into single strands is denaturation.

What is PCR?

PCR means polymerase chain reaction. It is a biological technique in molecular biology for creating multiple copies of DNA from a sample.

The stages involved in the polymerase chain reaction are as follows:

• Denaturation of the template into single strands
• Annealing of primers to each original strand for new strand synthesis
• Extension of the new DNA strands from the primers

Therefore, stage of PCR being represented by the the splitting of base pairs and the cleaving of DNA into single strands is denaturation.

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