A student observed a stained specimen of bacteria using bright-field microscopy. At 100x magnification, there appeared to be only one cell in the field of view, but at 1000x it was clear that there were two cells close together. The ability to distinguish these two cells as separate entities is called . View Available Hint(s) A student observed a stained specimen of bacteria using bright-field microscopy. At 100x magnification, there appeared to be only one cell in the field of view, but at 1000x it was clear that there were two cells close together. The ability to distinguish these two cells as separate entities is called . A.wavelength B.cell division C.resolution D.magnification

The ability of a microscope to distinguish between two separate points is known as resolution. if a microscope is focused at two separate points. but can only see them as a point then the microscope can not resolve or give details of the two points.Hence it has poor magnification. Thus the greater the amount of details of object showed by the microscope the higher the resolution e.g light microscope with resolution of 200nm. can not separate or resolve specimens where the distance of separations is more than 200nm. The reason fro this is that:

the limit of resolution is one-half of the wavelength of light of the microscope used to view it, if a specimen is smaller than half of the radiation used to view it then it can not be resolved by the microscope.

For example from last paragraph, the resolution of light microscope is 200nm, because the shortest wavelength of visible light is 400 nm, thus any specimens that is closer together than half of 400nm that is (200nm) can not be resolved by light microscope.

Electron microscopes have higher resolution than light microscope because they makes use of electrons instead of light. Electrons have grater wavelength therefore more energy,

and

they are negatively charged so they can easily be used to focus on an object by deflection of beam of electrons with magnetic in electromagnets component of the microscope for alerting the resolution.

With these features of electrons two types of electron microscope are the the scanning and transmission electron microscope

.Electron microscopes have resolution close to 0,5nm, hundred times grater than the light microscope because of the electron features.