What is the order of migration of hemoglobins acfs on cellulose acetate, ph 8.6, from the cathode to the anode?

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28-07-2022
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What is the order of migration of hemoglobins acfs on cellulose acetate, ph 8.6, from the cathode to the anode?

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sonalideval056 sonalideval056 · Answer 1 · 2022-08-06T17:47:21+02:00

Hb S separates from Hb D and Hb G but migrates with them on alkaline electrophoresis is the order of migration of hemoglobins acts on cellulose acetate, ph 8.6, from the cathode to the anode.

Explanation:

One of the oldest methods of protein panel analysis is cellulose acetate membrane electrophoresis (CAME).
Urinary protein electrophoresis has not been widely employed in clinical practice, and its principal use has been to validate the presence of the Bence Jones protein in multiple myeloma 6.
Some hemoglobins acquire a negative charge and move toward the anode in an acidic pH, whereas others acquire a positive charge and move toward the cathode (negative pole). For instance, on an acid electrophoresis, Hb S separates from Hb D and Hb G but migrates with them on alkaline electrophoresis.

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