ATP is the phosphoryl group donor
The free energy of the phosphorylation reaction is large.
Phosphorylation is known to be an effective way of regulating protein and in eukaryotic cells, about 30% pf the protein are phosphorylated. Thus, the enzyme responsible for the phosphorylation reaction is known as kinases. However, ATP is required in the process phosphorylation because it is needed as a donor.