When you added primary antibody to the wells, what happened if your sample contained the antigen? what if it did not contain the antigen? when you added secondary antibody to the wells, what happened if your sample contained the antigen? what if it did not contain the antigen? if the sample gave a negative result for the antigen, does this mean that the antigen is not present? what reasons could there be for a negative result when the antigen is actually present?

Question

03-04-2018
Biology

contestada

When you added primary antibody to the wells, what happened if your sample contained the antigen? what if it did not contain the antigen? when you added secondary antibody to the wells, what happened if your sample contained the antigen? what if it did not contain the antigen? if the sample gave a negative result for the antigen, does this mean that the antigen is not present? what reasons could there be for a negative result when the antigen is actually present?

Respuesta :

Otras preguntas

Thomas Jefferson as president is best remembered for: Select one: a. Leading the U.S. into a war with Britain b. Doubling the size of the United States by purch

U.S Government! Help Please!!! Which of the following is a true statement? A. The citizens of a state may only register to vote after proving to have submitted

Which best explains why Constantinople did not fall until 1453? The city was well protected and repelled attacks by invaders. The city created alliances with Eu

Which of the following best describes the significance of The Divine Comedy? A. The author wrote in Italian and presented very realistic characters. B. The char

Which of the following is an example of an X-linked recessive disorder? A. Huntington Disease B. Methemoglobinemia C. Color Blindness D. Sickle Cell Disease

Which of the following best describes the French intellectual Jean-Jacques Rousseau? A. a Romantic artist who painted scenes from nature B. a leader of the Fren

2/3 of what number is 16?

Solve each quadratic equation. Show your work. 14. (2x – 1)(x + 7) = 0

How did life change in the Soviet Union under Stalin?

The judicial power shall be vested in a supreme court, district courts of appeal, circuit courts, and county courts. The quote above is from the Florida constit

sharpguy sharpguy · Answer 1 · 2018-04-13T00:52:57+02:00

When I added a primary antibody to a well with a sample which contained the antigen, it bind to the antigen but could not bind with the antigen if it was not present for it would be flashed out in the wash step.

Adding secondary antibody, it produced a color change to the antigen because the enzyme bound secondary antibody, would bind to the primary antibody already bound to antigen in the wells. If the antigen was not present, there would be no binding in the wells thus the secondary antibody would have nothing to bid to and would be flashed out in the wash step.