HindIII and BamHI are the enzymes that will create a DNA
fragment that carries the entire vgp gene (shown in red) and has "sticky
ends"
Cloning the vgp gene requires an enzyme that has restriction
sites on both sides of that gene but not within the gene. Satisfying that requirement are the two
enzymes, namely HindIII and BamHI, while at the same time, creates DNA
fragments that contain sticky ends. Sticky ends make it possible for the
fragments to combine with the DNA of a cloning vector, such as a plasmid
BamH I, on the other hand is a type II restriction endonuclease, having the
capacity for recognizing short sequences (6 b.p.) of DNA and specifically cleaving them
at a target site.
