The UV/visible light must be absorbed by an electron before it can transition to an excited state orbital, which demands an increase in energy.
There are many variables that affect the outcomes of studies and absorbance measurements in UV visible spectroscopy. the following list of elements
The impact of the sample temperature
Sample concentration impact
pH impact on the sample
impact of the solvent
Effects of steric obstruction
Conjugation's impact
The location and shape of UV-Vis absorption bands are influenced by the sample solution's temperature, concentration, and pH. When the spectra are recorded at low temperatures, the absorption bands are sharp, whereas at high temperatures, the UV bands become broader.
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