The appearance of secondary granules and the first indication of neutrophilic differentiation can be seen in : Myelocyte.
Early granulocyte precursors (myeloblast and promyelocyte) appear similar across granulocytic cell lines until they reach the myelocyte stage, which is the final stage capable of cell division. They develop distinctive secondary lineage-specific granules at this stage (neutrophilic, eosinophilic or basophilic).
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White blood cells (leukocytes) go through the myelocyte stage of development where granules first show up in the cell cytoplasm. A precursor called a myeloblast transforms into a promyelocyte, which can be recognized by a nucleus that is slightly indented and shifted to one side of the cell.
When the promyelocyte cytoplasm fills with numerous granules, which may obscure the nucleus, the myelocyte stage develops. Hence, the first sign of neutrophilic differentiation and appearance of secondary granules is evident in myelocyte cell type.
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