adp and pi are added to the external medium and the vesicles are exposed to light, The DNP will collapse the H+ gradient that ATP synthase uses to generate ATP.
ATP synthase is the enzyme that makes ATP molecules. It is a multi-part complex that straddles the inner membrane of mitochondria, the energy factories in cells. The enzyme complex interacts with fatty molecules in the mitochondrial inner membrane, creating a curvature that is required to produce ATP more efficiently.
ATP can be hydrolyzed to ADP and Pi by the addition of water, releasing energy. ADP can be "recharged" to form ATP by the addition of energy, combining with Pi in a process that releases a molecule of water.
The DNP will collapse the H+ gradient that ATP synthase uses to generate ATP. Each of the three respiratory enzyme complexes includes metal atoms that are tightly bound to the proteins.
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