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Which of the following can be used to determine the rate of enzyme-catalyzed reactions?

1 -Rate of disappearance of the enzyme
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2 -Rate of disappearance of the substrate
3 -Rate of disappearance of the product
4 -Change in volume of the solution
5 - Increase in activation energy

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Answer:

Enzyme catalysis is detected by measuring either the appearance of product or disappearance of reactants. To measure something, you must be able to see it. Enzyme assays are tests developed to measure enzyme activity by measuring the change in concentration of a detectable substance.

The rate of disappearance of the substrate can be USED to determine the rate of enzyme-catalyzed reaction (Option 2).

  • A reaction is a chemical process where initial compounds referred to as 'substrates' are converted to different substances known as the 'products' of that reaction.

  • Enzymes are biomolecules (proteins) that bind to specific substrates and thus help to speed up the rate of a chemical reaction.

  • In conclusion, the rate of disappearance of the substrate can be USED to determine the rate of enzyme-catalyzed reaction (Option 2).

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