Culturing of the sputum resulted in the growth of distinct colonies on the medium, and the technician informs you that further isolation by subculturing is now needed. You understand that this is accomplished by taking a bit of growth from an isolated colony and inoculating a separate medium, resulting in the production of a_________.

A. broth.
B. pure culture.
C. mixed culture.
D. diagnosis.

Sputum sample contains varied types of bacteria.
When this sample is cultured on a growth medium all the different types of bacteria grow on it.
A single distinct colony of bacteria is formed from by multiple divisions of a single bacterial cell.
In the given growth medium distinct colonies of bacteria are formed.
Each such colony is formed from a single bacteria.
When such a single distinct colony is inoculated in a separate medium and bacteria is grown in that, then a Pure culture of bacteria is generated.
A Pure culture can be defined as such a culture which is comprised of single type of bacterial cell, formed from a single bacteria.