Question

The terms "EcoRI," "HaeIII," and "HindIII" refer to which of the following? (A) The voltage intensity used to prepare the electrophoresis medium (B) The restriction enzymes used (C) The organisms from which the original DNA sample was obtained (D) The types of buffers used to maintain a constant pH in the preparation as the sample was processed (E) The types of proteins encoded by each fragment

Answer 1

Answer:

The correct answer is - option B. the restriction enzyme used.

Explanation:

EcoRI, HaeIII and HindIII are the some of the restriction enzyme that are used in cleaving the DNA at specific site into fragments. It is also known as the molecular knife as it cleaves the DNA at molecular level.

EcoRI is isolated from E. coli., similarly HaeIII isolated from Haemophilus aegyptius, and Hind III isolated from Haemophilus influenzae.

Thus, the correct answer is - option B.

Answer 2

Answer:

The restriction enzymes used.

Explanation:

The recombinant DNA technology is the production of recombinant DNA and its use in the medicine and agriculture purpose. The rDNA technology uses DNA ligase and restriction endonuclease.

Restriction enzymes are known as molecular scissors used to cut the DNA molecules. The exonuclease cuts the DNA at the end and endonuclease cuts the DNA from inside. EcoRI," "HaeIII," and "HindIII" cuts the DNA molecules and acts as restriction enzymes.

Thus, the correct answer is option (B).