Question

For each of these cofactors, explain its chemical function and give an example of a particular enzyme that would use it: TPP, PLP, biotin, FAD, NADPH

Answer 1

Answer:

The function of the given cofactors are listed below.

Explanation:

TPP or thymine pyrophosphate   TPP is used as cofactor during Pentose phosphate pathway and de carboxylation of pyruvate.

PLP or pyridoxal phosphate        PLP is used as coenzymes in trans amination reaction.

Biotin or vitamin B7       Biotin is used as coenzyme  during one carbon transfer reaction For example pyruvate to oxaloacetate.

FAD or Flavin Adenine dinucleotide   FAD is used as co enzyme in many metabolic reactions such as fatty acid oxidation, TCA cycle.

NADPH   NADPH is used as coenzyme to minimize the toxic effect of reactive oxygen species.

   Co enzymes                                   Enzymes

1 TPP                                                1 Trans ketolase

2 PLP                                                2 Alanine  aminotransferase

3 Biotin                                             3 Pyruvate  carboxylase

4 FAD                                                4 Acyl  CoA dehydrogenase

5  NADPH                                          5 Glutathione  reductase.