A student receives the sequencing results for a bacterial enome. After analyzing the results, he is disappointed to ind a large gap in the alignment of his contigs. The student decides to obtain the missing sequence data via polymerase chain reaction (PCR). Gap Select the correct requirements for using PCR to determine the sequence of this gap. one primer must be complementary to the 3-beginning contig at the gap. Contig ends must lie close enough together for the reliable amplification of the entire length of the gap. One primer of the pair must not contain repeated genome sequences. At least one primer must be complementary to the 5-beginning contig at the gap. ? Gaps must be shorter than 200 bp in order to amplify accurately.

The ADN hebra is in 3-5 order, there aretwo of them, which means that both hebras haveto be with its pair, if one starts with 3 the other must connect with the 5 of the other one.

In order to make the PCR work, it must start with the opposite one as well and have it.

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