A researcher is performing a gram stain on a specimen in a medical laboratory. She gets distracted by a phone call and accidentally uses water instead of alcohol to decolorize. What may happen as a result of this mistake?
It wouldn't be able to separate negative gram from positive. it would not wash away some of the crystal violet stain effectively. Gram - Cells will appear to be the same hue. Water can also dilute cells or wash them off the slide
Would not be able to differentiate gram - from + it would not efficiently wash some of the crystal violet stain away. gram - cells will still remain the same color. also water may dilute or wash cells off the slide
