Question

The resolution calculated for the tlc separation of a two-component mixture is determined to be equal to 2.0. does the represent good separation or not? explain

Answer 1

Answer: No, it does not represent a good separation.

Explanation: Resolution is the measure of extent of separation between two components and the base-line separation. It is calculated using the formula

$R_s=2\times \frac{(Rt_1-Rt_2)}{(W_1+W_2)}$

Where, $R_s$ = resolution

$(Rt_1-Rt_2)$ = Difference between the retention times of two components.

$W_1+W_2$ = Spot widths of two components.

The perfect resolution is considered as 100.

Here, we are given that the two components in a mixture both have a resolution at 2.0 that means both the peaks are overlapping each other and thus the components cannot be determined accurately.

Thus, this does not represent a good separation as as the two components are spotted closely.