Question

Compare the dna fingerprint of person 1 and person 2. explain how this fingerprint would have looked different if you had digested the dna of each person with hindiii instead of ecori.

Answer 1

Person 1 and person 2 clearly have separate DNA fingerprints. This means they are different people. If the experimenter had digested the dna with hindiii instead of ecori, it would separate the strands of DNA differently, which would cause the DNA fingerprint itself to change.

Answer 2

EcoRI and HindIII recognize different sequences of DNI and cut the molecule in different points. For this reason, when digesting the molecule with HindIII, the exposed pattern of minisatellites would have looked different than when using EcoRI.

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A restriction enzyme is a degradative polymer that recognizes and cuts up specific nucleotide sequences in DNI. Restriction enzymes can produce cohesive cuts or blunt (abrupt) cuts, depending on the recognition sites. The frequency in which the restriction enzyme will produce the cuts depends on the recognition sequences and the number of bases that compose them.

• EcoRI cuts between G y A.
• Hind III cuts between A y A.

So restriction enzymes cut DNI molecules into different short sequences of varying lengths. These pieces are called minisatellites and exhibit the existing variation from one person to the next. These short sequences can be separated by using gel electrophoresis and will express a pattern of differentiation. These are the restriction fragment length polymorphisms pattern, RFLP. Each individual has a unique pattern of minisatellites, which makes this person different than the rest.

The method used to identify an individual based on the minisatellites patterns in their DNI is known as DNA fingerprinting. Although the difference in base sequences between two individuals is very small, fragments produced by restriction endonucleases are of different sizes.

Because EcoRI and Hind III recognize different sequences and cut sites, they will cut the same molecule in different pieces, resulting in a different pattern.  

So, when digesting DNI of person 1 and person 2 with

• EcoRI → the short sequences pattern will express certain differences between individuals.  
• HindIII → the exposed pattern will differ from the first one and show other differences.
• both enzymes together → minisatellites will be even shorter and express a third different pattern.  

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