Question

Using the equation of the line (y = 0.5x + 79), what would be the melting point of dna with 50% gc content? enter your answer as a whole number

Answer 1

We have the equation of the line as follows:
y = 0.5x + 79

We want to calculate the melting point (the y value) of DNA  with 50% gc content (the x value).
The gc content represents the value of x in the equation, so to calculate the melting point (y), we will simply substitute the x with 50 in the equation of the line as follows:
melting point = 0.5(50) + 79 = 25 + 79 = 104 degrees

Answer 2

The melting point of DNA with 50% GC content = 104 °C

Further explanation

DNA has a base pair of bonds between adenine and thymine and guanine and cytosine. These bonds are hydrogen bonds.

Three hydrogen bonds between guanine-cytosine and two hydrogen bonds adenine-thymine make denaturation of DNA by heating it requires greater heat energy.

The amount of guanine and cytosine in the DNA molecule (the GC content) is directly proportional to the melting point, the temperature at which DNA is denatured

If we plot between the melting point of DNA (y-axis) and the percentage of the GC content (x-axis) we will get a straight line equation

From the equation of the line (y = 0.5x + 79), with GC content 50%, we enter the GC value as x so we get the DNA melting point as y

y = 0.5.50 + 79

y = 104 °C

Learn more

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the structure of a nucleotide DNA and an RNA nucleotide.

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the antiparallel arrangement of DNA strands

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Keywords: GC content, DNA structure, melting point, hydrogen bonds, adenine, thymine, guanine, cytosine, denaturation