Question

Widespread use of this process became practical after finding a replacement for the Klenow fragment. Fluorescent dyes allow the quantitative form of this process to be performed in real time. This process makes use of an enzyme from Thermus aquaticus due to its thermostability. This process involves thermal cycling through its denaturing, annealing, and extension stages. This process, which was developed by Kary Mullis, uses Taq polymerase rather than DNA polymerase. For 10 points, name this biochemical technique used to amplify segments of DNA.
a) Western blotting
b) Southern blotting
c) Polymerase chain reaction (PCR)
d) Gel electrophoresis

Answer 1

Answer:

c) Polymerase chain reaction (PCR)

Explanation:

Because PCR fits all the criteria mentioned in the question: it involves thermal cycling through denaturing, annealing, and extension stages, it uses Taq polymerase from Thermus aquaticus, it was developed by Kary Mullis, and it allows for real-time quantitative analysis with fluorescent dyes.