A solution of a specific vitamin has ?max = 275 nm and a concentration of 2.71 × 10-7 M. The absorbance of the solution at 275 nm is A = 0.150. What is the molar absorptivity of the vitamin at 275 nm? (sample pathlength = 1.00 cm)

Respuesta :

To solve this problem, we use the Beer-Lambert's Law this relates absorbance and concentration of the species that is absorbing light. It is expressed as A = eCl where e is the molar absorptivity, C is the concentration and l is the path length.

A = eCl
0.150= e(2.71x10^-7 M)(1.00 cm)
e = 553505.5351 L / mol-cm

Note:        A = 0,150

                 C = 2.71 x 10-7 M

                  l = 1.00 cm

Asked:       e =?

Answer: The formula is:

               A = eCl

         0,150 = e (2.71 x 10-7 M) (1.00 cm)

                e = 553505.53 L / mol-cm

Thus, the vitamin molar absorption at 275 nm is 553505.53 L / mol-cm

Further Explanation

The mass is a physical property of an object that is used to explain the various behaviors of the object being monitored. In everyday use, mass is usually synonymous with weight. But according to modern scientific understanding, the weight of an object is caused by the interaction of mass with the gravitational field.

Mol is a unit of measurement in the International Unit System (SI) for the number of substances. This unit is defined as the number of chemicals containing the number of representative particles, for example, atoms, molecules, ions, electrons, or photons, which is equivalent to the number of atoms in 12 grams of carbon-12 (12C), carbon isotopes with definitive standard atomic weight 12. Number this is expressed as an Avogadro number, with an approach value of 6.022140857 × 1023 mol − 1. Moles are one of the basic units of SI and are denoted by moles.

Molar mass, symbol (M), is the mass of one mole of an element or chemical compound. He is the physical nature of each pure compound. The SI unit for mass is kilograms, but for practical and historical reasons, the molar mass is almost always written in grams per mole (g / mol), mainly in chemistry. Molar mass is closely related to the relative molar mass (Mr) of a compound and is also related to the standard atomic weight of the constituent elements of the compound. However, the molar mass must be distinguished from molecular mass, which is the mass of one molecule and is not directly related to the atomic mass or relative atomic mass.

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Grade: High School

Subject: Chemistry

keywords: Molar mass

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