Use the following information to calculate transformation efficiency. a. You put 10 microliters of 0.03 micrograms/microliter PGLOTM solution into the +DNA tube. Calculate the micrograms of DNA you put into the tube. 10 microliters 0.03micrograms = 0.3 micrograms 1 microliter b. The +DNA tube contained -500 microliters of solution prior to plating, but you did not use all of it. Calculate the fraction of the DNA solution you used on each plate. 10microliters/500microliters = 0.02/plate c. Use your answers to questions 8a and 8b to calculate the micrograms of DNA you plated. 0.3micrograms x 0.02 micrograms -0.006 micrograms
