Two NADPH molecules were also produced during the entire glycolysis process. Six ATP molecules are created during glycolysis by NADPH, or one NADH yields three ATP molecules. Because 2 ATP molecules were used up in the earlier phases, the total amount of ATP molecules produced is 10 ATP, leaving a net gain of 8 ATP molecules.
For every mole of glucose, 2 ATP are produced (net) in anaerobic conditions. While glycolysis generates seven ATP for every mole of glucose generated, lactate dehydrogenase converts pyruvate to lactate anaerobically at a cost of two NADH (5 ATP). 2 ATP is equal to 7 ATP minus 5 ATP.
To maintain the NAD+ reservoir full, the two NADH molecules generated during glycolysis are oxidised during lactic fermentation. In this mechanism, each glucose molecule yields just two ATP molecules.
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