Yes, sterically shielded, stabilized nitrile imine can be used for rapid bioorthogonal protein labeling in live cells.
This type of nitrile imine is able to react quickly and efficiently with bioorthogonal molecules such as azides or alkynes, making it ideal for labeling proteins in live cells. It also has increased stability, which allows it to remain active for a longer period of time, and its shielding capabilities ensure that it does not interfere with the cell's normal processes.
Sterically shielding nitrile imine reduces its hydrophobicity, allowing it to remain in the aqueous environment of the cell and interact with target proteins. Together, these modifications make nitrile imine a powerful tool for labeling proteins in live cells.
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