Exhibit 2B Contains information on the pK's of some common buffers. Buffer pK1 pK2 pK3 Acetate 4.75 Ammonia 9.25 Carbonic acid 6.37 10.20 Citric acid 3.09 4.75 5.41 Formic Acid 3.75 Phosphoric acid 2.14 7.20 12.4 Pyruvic acid 2.50 Tris 8.3 Which of the following is important to know when deciding if a given buffer will be effective for an experiment? O the pk, of the buffer compound O the buffer capacity O the concentration of the buffer O whether the experiment is likely to generate hydrogen ions or hydroxide ions O all of these are important considerations

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Understanding acetate can help you determine whether a certain buffer will be useful for your experiment. The buffer compound's pk is O. Is the buffer capacity O.

The buffer capacity is crucial to the effectiveness of a buffer for an experiment. The buffer maintains the pH while the experiment proceeds within a reasonable pH range, which is vital. The buffer's capacity determines the appropriate pH range. When the proportions of acid and conjugate base are roughly equal, or the maximum buffer capacity, a buffer performs well.

Although buffers are immune to pH fluctuations, they are not impervious. The pH of a buffer will significantly vary if you add enough acids or bases.

Remember that a buffer is a weak acid and that its conjugated base is why this occurs in order to understand why (or weak base and its conjugated acid).

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