When two compounds might have spots with the same Rf value in a TLC experiment and not be identical compounds then repeating the original experiment with a different solvent system might help in determining whether the compounds are identical.
Chromatography is a separation technique which is used in the laboratory to separate two different substances physically.
In thin layer chromatography (TLC), two phases are involved viz. stationary phase and mobile phase. For this technique, the TLC plate is coated with silica gel which serves as the stationary phase. The solvent that is eluded into the stationary phase is known as the mobile phase.
Rf value or the retention factor is defined as the ratio of the distance travelled by the compound to the distance travelled by the solvent.
It is represented as:
Rf = distance travelled by compound/ distance travelled by solvent
To know more about TLC here
https://brainly.com/question/28941884
#SPJ4
