Ligase catalyzes a reaction between the 5'-phosphate and the 3'-hydroxyl at the ends of DNA molecules. The enzyme calf intestinal phosphatase catalyzes the removal of the 5'-phosphate from DNA molecules.
What would be the consequence of treating the vector, before ligation, with calf intestinal phosphatase?
A) It would prevent the ends of the plasmids from being ligated.
B) It would prevent the plasmid's DNA from reversing polarity during ligation.
C) It would reverse the polarity of plasmids' DNA
D) It would ligate the ends of the plasmids.A) It would prevent the ends of the plasmids from being ligated.

Ligase catalyzes a reaction between the 5'-phosphate and the 3'-hydroxyl at the ends of DNA molecules. consequence of treating the vector, before ligation, with calf intestinal phosphatase would prevent the ends of the plasmids from being ligated.

Ligation is the fusion of two nucleic acid pieces by an enzyme in molecular biology. It is a crucial laboratory procedure in DNA cloning in which DNA fragments are joined to form recombinant DNA molecules (such as when a foreign DNA fragment is inserted into a plasmid). Phosphodiester bonds are formed between the 3'-hydroxyl of one DNA terminus and the 5'-phosphoryl of another to join the ends of DNA fragments. RNA can also be ligated in the same way. In most cases, a co-factor, such as ATP or NAD+, is involved in the reaction.

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