For each genotype, indicate the state of the lactose operon if lactose is, or is not, provided in the medium. Partial diploids are achieved by additional lac genes carried by the F factor. Note: OC renders the operator insensitive to the repressor, I- indicates a defective repressor, and Is is a mutation that makes the repressor insensitive to lactose. Constitutive = always transcribed; Induced = up-regulated; Repressed = down-regulated.Genotype/medium Constitutive Induced RepressedIsOCZ+: No Lactose IsOCZ+: Lactose IsO+Z+: No Lactose IsO+Z+: Lactose

Respuesta :

Lactose is present, but glucose is not: strong transcription of the lac operon occurs. Because the inducer (allolactose) is present, the lac repressor is released from the operator.

Because glucose is not present, cAMP levels are high, indicating that CAP is active and bound to the DNA.  It's expressed only when lactose is present and glucose is absent.

in this case we conclude by these terms

I- = always on

Oc = always on

Z- = no lactose

Is = super repressor = always off, can only be inhibited by Oc

Examples:

W/ lactose & w/out lactose

F'-I+/I-O+Z- no & no

F'-Is/I-OcZ+. yes & yes

I +O+Z+/F' I-O+Z+. yes & no

I-OcZ-/F' I-Oc Z- no no

I-OcZ+/F' I-O+Z+. yes & yes

IsOcZ-/F' IsO+Z+. no & no

Without the regulator repressor, enzyme production will be constitutive;

enzyme production is still repressible.

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