Carbon Dioxide.
A presumptive test called phenolphthalein reacts with the heme molecule found in blood. Pink is produced when a reaction is favorable. While bloodstains typically have a reddish-brown hue, the color of the substrate or the stain's age can have an impact on how visible or noticeable the stain is.
A technique for separating substances or metal complexes based on their relative solubilities in two different immiscible liquids, typically water (polar) and an organic solvent, is known as liquid-liquid extraction (LLE), also known as solvent extraction and partitioning (non-polar).
Precipitin Test confirmation is required for positive results. The serologist must determine whether a stain is actually blood after a presumptive test suggests it might be blood. A precipititin test can detect proteins that are only present in human blood.
The Ouchterlony test compares the responses of a blood sample to particular antibodies to identify if it is human or animal. A sample of the unidentified bloodstain is put in an agar gel well.
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