Two substrates are transported across the membrane through anti ports that are hostile to one another.
For every Ca2+ molecule transported by the Na+/Ca2+ antiport, three Na+ molecules are also brought into the cell. The Na+ enters the cell thanks to the electrochemical gradient. The energy from Na+ moving down its electrochemical gradient is used to propel Ca2+ against its gradient as it is pushed out of the cell. This keeps the cell's cytoplasmic Ca2+ level extremely low. The amount of Ca2+ in the cytosol affects how forcefully the heart muscles contract. Moving a protein from one cellular or extracellular compartment to another is known as protein transport. Numerous mechanisms, such as membrane trafficking, protein translocation, and endocytosis or exocytosis, can aid in the transport of proteins.
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