after isolating a plasmid vector and a gene of interest, what is the order of steps for producing a cell with a new gene through recombinant dna technology (i.e., transformation)? transform into host cell

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16-11-2022
Biology

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after isolating a plasmid vector and a gene of interest, what is the order of steps for producing a cell with a new gene through recombinant dna technology (i.e., transformation)? transform into host cell

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qwbeige qwbeige · Answer 1 · 2022-11-24T12:22:10+01:00

The steps to introduce a new gene into a host cell involves extraction of DNA from the desired individual and then introducing it into the host through vectors.

A target gene is often put onto a plasmid, a circular piece of DNA, in a cloning process. Bacterium harbouring the plasmid are chosen using antibiotics after the plasmid is introduced into the bacteria through a procedure known as transformation. DNA "cut and paste" enzymes are used for the insertion, which results in a molecule of recombinant DNA, or DNA that has been put together from fragments from various sources.

The following steps make up the recombinant DNA technology process:

extraction of genetic information (DNA)
DNA is cut in precise places.
connecting DNA fragments.
DNA insertion within the host cell.
choosing and testing transformed cells.

To know more about recombinant DNA technology, refer to the following link:

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