The correct answer :
Flanking Seq are regions before and after transcribed region. At least one of the flanking sequences you selected is not correct. When a transposon recognizes a target sequence, the target sequence is cut to generate overhanging single-strand ends. After the transposon inserts between this cut region, DNA polymerase fills in the single‑stranded regions and thus duplicates the original target sequence.
Consider that because the insertion occurs in double‑stranded DNA there is also a complementary strand. No other change typically occurs to the sequence during this process. A simple or conservative transpose is a non-replicating transpose. That is, in conservative rearrangements transposons are completely removed from the genome and reintegrated into new non-homologous loci preserving the same gene sequence throughout the process.
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