If the sample's absorbance is higher than 1.2, we must contact our TA to decide whether new calibration standards need to be made or whether existing standard concentrations need to be changed.
Here is an example of how to use Beer's Law directly when you know the molar absorptivity constant: Absorbance = e L c. (or molar extinction coefficient). The molar extinction coefficient, e, is used in this equation. L is the cell holder's path's length. The solution's concentration is given by c.
The concentration of particular compounds in a solution can be ascertained by measuring the absorbance of the solution. A solution's purity can also be determined using absorbance.
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