We can say that prokaryotes, such as bacteria differ from eukaryotes in some aspects, between them we could say that prokaryotes do not have membrane-bound organelles like eukaryotes have, so they do not have a nucleus either, since this is a membranous structure. Their genetic material, which is circular, is free on their cytoplasm in usually one chromosome, so they do package their DNA in these structures. But they are single-celled organisms that can live in colonies, though. So the correct answer is the second one.
