Question

A student who was trying to determine the concentration of escherichia coli in a sample completed a total plate count assay. After plating 1ml of the 10-6dilution tube and incubating the plate at 37oc for 24 hours the student counted 210 colonies on the plate. What is the cfu/ml of the original sample?.

Answer 1

From the total plate count assay, the  colony forming units/ml (CFU/ml) of the original sample is 2.1 * 10⁸ CFU/mL.

What is the Colony Forming Units/ml (CFU/ml) of the original sample?

The the Colony Forming Units/ml (CFU/ml) of a given sample of of cultured bacteria is a measure of the number of colonies formed per given mL f the final diluted sample of the cultured bacteria.

The formula for calculating the Colony Forming Units/ml (CFU/ml) of a given cultured bacteria sample is given as follows:

• CFU =  number of colony forming units on the countable plate * 1/FDF

Where:

FDF is the final dilution factor

The final dilution factor is the product of all the dilution factors from the original sample to the countable plate.

From the dat provided:

FDF = 1 * 10⁻⁶

number of colony forming units on the countable plate = 210

The colony forming units/ml (CFU/ml) of the original sample = 210 * 1/1 * 10⁻⁶

The colony forming units/ml (CFU/ml) of the original sample = 2.1 * 10⁸ CFU/mL

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