Homogenization, salt fractionation, column chromatography, electrophoresis.
Monitoring the process at each stage by identifying particular activity and carrying out an SDS-PAGE analysis can help you establish whether a protein purification strategy is successful. The following variables are monitored at each step:
Entire protein: The amount of protein in a fraction is calculated by multiplying the protein concentration of a portion of each fraction by the total volume of the fraction.
Entire activity: The enzyme activity in the volume of the fraction utilized in the experiment is calculated by multiplying the enzyme activity by the total volume of the fraction.
For instance, if protein purification procedures like homogenization, salt fractionation, Ion Exchange chromatography, Gel filtering, and Affinity chromatography are used.
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