When an E. Coli cells are grown in presence of both glucose and lactose, the lac operon is not expressed. This condition is known as negative control.
As we know, if both glucose and lactose is present, an E. Coli will utilize only glucose as a source energy and lactose is reserved as alternative source. Due to which lac operon is not expressed, and this condition is commonly known as negative control.
Therefore, Wild E. Coli are grown in presence of glucose and lactose because E. coli will use only glucose, not the lactose, and lac operon which required for transport and metabolism of lactose in the E.coli is absent or not expressed.
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