Bacillus subtilis is an aerobic, Gram-positive soil bacterium that has been frequently employed in the manufacture of heterologous proteins. It secretes several enzymes to break down a wide range of substrates, allowing the bacteria to thrive in a constantly changing environment.
This species and some of its close relatives have exceptional protein secretion ability, making them valuable hosts for the manufacture of therapeutic proteins and industrial enzymes.
For these reasons, it has been widely utilized to create heterologous proteins. Furthermore, it has outstanding physiological features and a highly adaptable metabolism, making it easier to produce on low-cost substrates.
Its varied protein secretion systems, as well as the recently created artificial promoter and ribosome binding site (RBS) libraries, are also useful in the creation of extracellular enzymes.
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