Two proteins that differ greatly in size (molecular weight) but not overall charge would best be separated by?

Gel filtration chromatography, a type of size exclusion chromatography, can be used to either eliminate all molecules larger than a certain size from a sample or to segregate molecules and complexes in a sample into fractions with that size range.

Gel filtration chromatography may be used to separate substances including tiny molecules, proteins, protein complexes, polysaccharides, and nucleic acids from aqueous solutions. When an organic solvent is used as the mobile phase, the technique is known as gel permeation chromatography.

The following are other uses for gel filtration chromatography:

molecular and complex separation within a certain size range
Radioisotope separation into bound and unbound states eliminates
analysis and size determination of big proteins and complexes.

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