3-phosphoglycerate is the product formed in the first substrate level phosphorylation in glycolysis.
- A cytoplasmic mechanism called glycolysis converts glucose into two molecules with three carbons each and releases energy.
- Hexokinase, a phosphorylating enzyme, assists in the phosphorylation process that traps glucose.
- This reaction uses adenosine triphosphate (ATP), and the end result, glucose-6-P, inhibits hexokinase.
- In contrast to oxidative phosphorylation, which produces ATP from ADP and an inorganic phosphate, Pi, substrate-level phosphorylation produces ATP from ADP and a phosphorylated intermediate.
- The amount of ATP produced by glycolysis is not very high.
- At the first substrate level of glycolysis, 3-phosphoglycerate is created from 1,3-bisphosphoglycerate
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