There would be very little or no DNA produced if in a pcr reaction you used a dna polymerase isolated not from thermus aquaticus, but from an organism that grows at lower temperature .
What is the purpose of PCR?
To amplify (copy) a gene so it can be detected.
How do the strands separate during PCR?
- The hydrogen bonds between the two strands are broken by the DNA polymerase.
- During the annealing process, the primers split the strands. - The hydrogen bonds between the two strands are broken by the high heat of the denaturation stage.
What is a PCR test ?
- Targeting particular DNA fragments and amplifying (increasing in number) them artificially is done using the polymerase chain reaction technology. Describe how primers are used in PCR.
- The primer is a synthetic DNA strand that has bases that are complementary to the bases at the start of the DNA fragment that will be amplified.
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