We would not expect see the pale color in presence of inhibitor in PNPP-PNP reaction. PNPP ( P- nitrophenylphosphate) is chromogenic substance for alkaline phosphate , which is clear yellow color. PNP (P- nitro-phenol ) is a colorless and acidic solution.
PNP is stronger acid and it is used as a manufacture drugs, fungicies, insecticides and dyes . Over inhalation of 4-nitro-phenol can causes headache, drowsiness , nausea, and cyanosis.
PNPP is used for detecting alkaline phosphate in ELISA applications. It sis non proteinaceous non -specific substance used to assay protein , alkaline and acid phosphates. And its yellow color is because of maximum of absorbance at 405 nm.
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