In a tube of media, Obligate anaerobes would be found at the very bottom of the media.
These microbes are poisoned by oxygen, so they gather at the bottom of the tube where the oxygen concentration is lowest.
Obligate anaerobes do not possess the defenses that make aerobic life possible and therefore cannot survive in air. The excited singlet oxygen molecule is very reactive. Therefore, superoxide must be removed for the cells to survive in the presence of oxygen.
Microaerophiles will grow in a thin layer below the richly-oxygenated layers.
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