A mutation has occurred directly after the start codon within an mrna sequence. A deletion of two nucleotide provided mutation will have the most serious effect on the resulting polypeptide.
The first codon in mRNA transcript is known as start codon.
Start codon provides initiation signals for the ribosomes to start the process of translation during which formation of polypeptide chain takes place.
It arises when the normal sequence of codon is disrupted by the insertion or deletion of one or more nucleotides, provided that the no. of nucleotide which is added or removed is not multiple of three.
Thus, we concluded that the deletion of two nucleotide make frameshift mutation.
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