Question

A researcher has sequenced a gene that is present in two alleles. She has a mixture of both alleles and clones them into e. Coli using plasmids that contain the lacz gene. She is interested in one particular allele that is indicated in a disease. Using media containing x-gal, how can she select those colonies that have her allele of interest?.

Answer 1

She will have to sequence the plasmids of the white colonies before knowing which allele it contains.

What are plasmids?

• A plasmid is a tiny, circular, double-stranded DNA molecule that differs from chromosomal DNA in a cell.
• Plasmids are naturally found in bacterial cells and in certain eukaryotes. plasmid genes frequently confer genetic benefits to bacteria, such as antibiotic resistance.
• RNA plasmids are uncommon, and the majority of them are poorly understood.
• Plants, fungi, and even mammals have provided examples. Linear RNA plasmids are found in some strains of the yeast Saccharomyces cerevisiae.
• Similar RNA plasmids have been discovered in the mitochondria of various maize plant types.

To learn more about plasmids from the given link

https://brainly.com/question/2088910

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Answer 2

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