The subtrate will no longer fit within the enzyme because the heat will cause the enzyme to denature (deform).
Enzyme
Proteins called enzymes serve as biological catalysts (biocatalysts). Chemical reactions are sped up by catalysts. Substrates are the molecules that enzymes can interact with, and the enzyme changes the substrates into new molecules known as products. Almost all cellular metabolic activities require enzyme catalysis in order to proceed at speeds quick enough to maintain life. Enzymes are necessary to catalyze specific steps in metabolic pathways. Enzymology, the study of enzymes, and the pseudoenzyme analysis area acknowledge that some enzymes have lost the capacity to perform biological catalysis during evolution, which is frequently reflected in their amino acid sequences and peculiar "pseudocatalytic" capabilities.
Students are measuring the rate of enzyme activity. The graph below shows the rate of enzyme action as they increase the temperature
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