A final statement or concluding statement
In the dna isolation process, Meat Tenderizer is used to break down the protein complexes and allow the dna molecules to easily precipitate.
Deoxyribonucleic acid (DNA ) isolation is an extraction process of DNA from various sources. Methods used to isolate DNA are dependent on the source, age, and size of the sample. Despite the wide variety of methods used, there are some similarities among them. In general, they aim to separate DNA present in the nucleus of the cell from other cellular components.
Three steps are involved in DNA isolation:
1. Lysis: This is the mechanical breakdown of the cell during which the DNA is released from the nucleus. Enzymes and detergents like Proteinase K must be used throughout this process.
2- Precipitation: During this step, salt and alcohol are used to precipitate the DNA.
3. Purification: This step rids DNA of contaminants. DNA is extracted by being cleaned with ethanol and water.
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