What outcome results from improper washing of a tube or well after adding the enzyme–antibody conjugate in an elisa system? Result will be falsely increased
The enzyme-linked immunosorbent assay (ELISA), first described by Eva Engvall and Peter Perlmann in 1971, is a widely used analytical biochemistry technique. The assay employs a solid-phase type of enzyme immunoassay (EIA), utilizing antibodies against the target protein to find the presence of a ligand (often a protein) in a liquid sample. In addition to being utilized as a quality control check in numerous industries, ELISA has been used as a diagnostic tool in biotechnology, plant pathology, and medicine.
Antigens from the sample to be examined are coupled to a surface in the simplest ELISA. The surface is then covered with a corresponding antibody so it can bind the antigen.
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