During synthesis of triacylglycerols from free fatty acids Acetyl-CoA is least important in this synthesis.
Acetyl-CoA would not be important because thats what that product is based from the synthesis,
The biosynthesis of triacylglycerol occurs in the endoplasmic reticulum (ER) and involves acyl-editing of fatty acyl chains within the nitrogenous phospholipids of the ER.
The enzyme glycerol kinase has no role to play in the synthesis of triacylglycerol.
Pancreatic enzymes called lipases then hydrolyze the dispersed fats to give monoglycerides and free fatty acids. These products are absorbed into the cells lining the small intestine, where they are resynthesized into triglycerides.
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