A very high WBC count can lead to turbidity in the cyanmethemoglobin method resulting in falsely high hemoglobin value.
Cyanmethemoglobin method is a method which is used for the estimation of hemoglobin. In this method, hemoglobin is converted to cyanmethemoglobin by adding ferricyanide and potassium cyanide. The absorbance is then measured at 540nm using a spectrophotometer.
This is a very advantageous method as it is able to measure all forms of hemoglobin except sulphaemoglonin and also since spectrophotometer is used, subjective errors are avoided. But, if the WBC count is very high, it can lead to turbidity in the cyanmethemoglobin method resulting in falsely high hemoglobin value.
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